Romig, T. et al. Elsevier Academic Press Inc, San Diego,. Ecology and life cycle patterns of Echinococcus species. in Echinococcus and Echinococcosis, Part A (eds. Thompson, R. C. A., Deplazes, P. & Lymbery, A. J.), Adv. Parasitol., 95, 213–314 (2017).
Torgerson, P. R., Keller, K., Magnotta, M. & Ragland, N. The global burden of alveolar echinococcosis. PLoS Negl. Trop. Dis. 4, e722 (2010).
FAO/WHO. Multicriteria-Based Ranking for Risk Management of Food-Borne Parasites. 23, 1–302. (2014).
Delcourt, J., Brochier, B., Delvaux, D., Vangeluwe, D. & Poncin, P. Fox Vulpes vulpes population trends in Western Europe during and after the eradication of rabies. Mammal Rev. 52, 343–359 (2022).
Bradley, C. A. & Altizer, S. Urbanization and the ecology of wildlife diseases. Trends Ecol. Evol. 22, 95–102 (2007).
Liccioli, S., Giraudoux, P., Deplazes, P. & Massolo, A. Wilderness in the ‘city’ revisited: different urbes shape transmission of Echinococcus multilocularis by altering predator and prey communities. Trends Parasitol. 31, 297–305 (2015).
Oro, D., Genovart, M., Tavecchia, G., Fowler, M. S. & Martínez-Abraín, A. Ecological and evolutionary implications of food subsidies from humans. Ecol. Lett. 16, 1501–1514 (2013).
Hegglin, D., Bontadina, F. & Deplazes, P. Human-wildlife interactions and zoonotic transmission of Echinococcus multilocularis. Trends Parasitol. 31, 167–173 (2015).
Oksanen, A. et al. The geographical distribution and prevalence of Echinococcus multilocularis in animals in the European union and adjacent countries: a systematic review and meta-analysis. Parasit. Vectors. 9, 23 (2016).
Deplazes, P., Hegglin, D., Gloor, S. & Romig, T. Wilderness in the City: the urbanization of Echinococcus multilocularis. Trends Parasitol. 20, 77–84 (2004).
Manfredi, M. T., Genchi, C., Deplazes, P., Trevisiol, K. & Fraquelli, C. Echinococcus multilocularis infection in red foxes in Italy. Vet. Rec. 150, 757–757 (2002).
Citterio, C. V. et al. Echinococcus multilocularis and other cestodes in red foxes (Vulpes vulpes) of Northeast Italy, 2012–2018. Parasit. Vectors 14(1), 1–12 (2021).
Obber, F. et al. A highly endemic area of Echinococcus multilocularis identified through a comparative re-assessment of prevalence in the red Fox (Vulpes vulpes), Alto adige (Italy: 2019–2020). PloS One. 17(5), e0268045, 1–12 (2022).
Massolo, A. et al. Unexpected Echinococcus multilocularis infections in shepherd dogs and wolves in south-western Italian Alps: A new endemic area? Int. J. Parasitol. -Parasit Wildl. 7, 309–316 (2018).
Casulli, A. et al. Multi-locus microsatellite analysis supports the hypothesis of an autochthonous focus of Echinococcus multilocularis in Northern Italy. Int. J. Parasitol. 39, 837–842 (2009).
Jones, A. & Pybus, M. J. Taeniasis and echinococcosis. in Parasitic Diseases of Wild Mammals. (eds Samuel, W. M., Pybus, M. J., Kocan, A. A., Pybus, M. J. & Kocan, A. A.) 150–192 (Iowa State University, Iowa, Iowa, (2008).
Laranjo-Gonzalez, M. et al. Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: Western Europe. Parasit. Vectors. 10, 1–14 (2017).
Deplazes, P., Eichenberger, R. M. & Grimm, F. Wildlife-transmitted Taenia and Versteria cysticercosis and coenurosis in humans and other primates. Int. J. Parasitol. -Parasit Wildl. 9, 342–358 (2019).
Torgerson, P. R. One world health: socioeconomic burden and parasitic disease control priorities. Vet. Parasitol. 195, 223–232 (2013).
Woolsey, I. D. & Miller, A. L. Echinococcus granulosus sensu Lato and Echinococcus multilocularis: A review. Res. Vet. Sci. 135, 517–522 (2021).
Loi, F. et al. Prevalence Estimation of Italian ovine cystic echinococcosis in slaughterhouses: A retrospective bayesian data analysis, 2010–2015. PLoS One. 14(4), e0214224, 1–19 (2019).
La Morgia, V. et al. Stima della distribuzione e consistenza del lupo a scala nazionale 2020/2021. 1–16 (2022).
Agnoletti, M. The degradation of traditional landscape in a mountain area of Tuscany during the 19th and 20th centuries: implications for biodiversity and sustainable management. Ecol. Manage. 249, 5–17 (2007).
Tomassini, O. et al. Some like it burnt: species differences in small mammal assemblage in a mediterranean basin nearly 3 years after a major fire. Mamm. Res. 69, 283–302 (2024).
Rapetti, F. & Vittorini, S. I caratteri del clima. in La Pianura Di Pisa E I Rilievi Contermini. (ed. M., M.) vol. 50, 103–132 (Roma, 1994).
Bertacchi, A., Sani, A. & Tomei, P. E. La Vegetazione Del Monte Pisano (Dipartimento di Agronomia e Gestione dell’Agroecosistema – Università di Pisa, Provincia di Pisa, 2004).
Veit, P. et al. Influence of environmental factors on the infectivity of Echinococcus multilocularis eggs. Parasitology 110, 79–86 (1995).
Mathis, A., Deplazes, P. & Eckert, J. An improved test system for PCR-based specific detection of Echinococcus multilocularis eggs. J. Helminthol. 70, 219–222 (1996).
Nakao, M., Sako, Y. & Ito, A. Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis. Infect. Genet. Evol. 3, 159–163 (2003).
Hüttner, M. et al. Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda: Taeniidae) from the African Lion. Int. J. Parasitol. 38, 861–868 (2008).
Dumendiak, S. et al. Hidden diversity of cestodes in wild African carnivores: I. Non-taeniid cyclophyllideans. Int. J. Parasitol. -Parasit Wildl. 24, 1–13 (2024).
Santa, M. A., Pastran, S., Klein, C., Ruckstuhl, K. & Massolo, A. Evaluation of an automated magnetic bead-based DNA extraction and real-time PCR in fecal samples as a pre-screening test for detection of Echinococcus multilocularis and Echinococcus Canadensis in Coyotes. Parasitol. Res. 118, 119–125 (2019).
Klein, C., Liccioli, S. & Massolo, A. Egg intensity and freeze-thawing of fecal samples affect sensitivity of Echinococcus multilocularis detection by PCR. Parasitol. Res. 113, 3867–3873 (2014).
Knapp, J. et al. Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. Vet. Parasitol. 201, 40–47 (2014).
Knapp, J., Umhang, G., Poulle, M. L. & Millon, L. Development of a Real-Time PCR for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of Toxocara spp. and Echinococcus multilocularis. Appl. Environ. Microbiol. 82, 2950–2958 (2016).
Brown, L. D., Cai, T. T. & Dasgupta, A. Interval Estimation for a binomial proportion. Stat. Sci. 16, 101–133 (2001).
Otero-Abad, B., Armua-Fernandez, M. T., Deplazes, P., Torgerson, P. R. & Hartnack, S. Latent class models for Echinococcus multilocularis diagnosis in foxes in Switzerland in the absence of a gold standard. Parasit. Vectors. 10, 1–14 (2017).
Manske, M. GENtle, a free multi-purpose molecular biology tool. Mathematisch-Naturwissenschaftlichen Fakultät (Universität zu Köln, (2006).
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35, 1547–1549 (2018).
Gori, F. et al. The occurrence of taeniids of wolves in Liguria (northern Italy). Int. J. Parasitol. -Parasit Wildl. 4, 252–255 (2015).
Fiocchi, A. et al. Helminth parasites of the red Fox Vulpes vulpes (L., 1758) and the Wolf Canis lupus italicus Altobello, 1921 in Emilia-Romagna, Italy. Ital. J. Zoolog. 83, 503–513 (2016).
Magi, M., Guardone, L., Mignone, W., Prati, M. C. & Macchioni, F. Intestinal helminths of red foxes (Vulpes vulpes) in north-west Italy. Helminthologia 53, 31–38 (2016).
Bariselli, S. et al. Identification and phylogenetic analysis of Taenia spp. Parasites found in wildlife in the Emilia-Romagna region, Northern Italy (2017–2022). Int. J. Parasitol. -Parasit Wildl. 22, 20–27 (2023).
Calderini, P. et al. Investigation on the occurrence of Echinococcus multilocularis in central Italy. BMC Vet. Res. 5: 44, 1–6 (2009).
Magi, M. et al. Endoparasites of red Fox (Vulpes vulpes) in central Italy. J. Wildl. Dis. 45, 881–885 (2009).
Poglayen, G. et al. Italian wolves (Canis lupus italicus Altobello, 1921) and molecular detection of taeniids in the Foreste casentinesi National park, Northern Italian apennines. Int. J. Parasitol. -Parasit Wildl. 6, 1–7 (2017).
Macchioni, F. et al. Taeniid cestodes in a Wolf pack living in a highly anthropic hilly agro-ecosystem. Parasite 28, 1–8 (2021).
Crotti, S. et al. Tapeworms detected in Wolf populations in central Italy (Umbria and Marche regions): A long-term study. Int. J. Parasitol. -Parasit Wildl. 21, 11–16 (2023).
Maurelli, M. P. et al. Innovative tools for the diagnosis of Echinococcus granulosus in definitive hosts. Parasitol. Res. 117, 2607–2612 (2018).
Kolapo, T. U. et al. Copro-polymerase chain reaction has higher sensitivity compared to centrifugal fecal flotation in the diagnosis of taeniid cestodes, especially Echinococcus spp, in Canids. Vet. Parasitol. 292, 1–5 (2021).
Guerra, D., Hegglin, D., Bacciarini, L., Schnyder, M. & Deplazes, P. Stability of the Southern European border of Echinococcus multilocularis in the Alps: evidence that Microtus arvalis is a limiting factor. Parasitology 141, 1593–1602 (2014).
Beerli, O., Guerra, D., Baltrūnaitė, L., Deplazes, P. & Hegglin, D. Microtus arvalis and Arvicola scherman: key players in the Echinococcus multilocularis life cycle. Front. Vet. Sci. 4, 1–10 (2017).
Mori, K., Liccioli, S., Marceau, D. & Massolo, A. A community analysis approach to parasite transmission in multi-host systems: assemblages of small mammal prey and Echinococcus multilocularis in an urban area in North America. Int. J. Parasitol. -Parasit Wildl. 9, 49–55 (2019).
Chaignat, V. et al. The brown hare (Lepus europaeus) as a novel intermediate host for Echinococcus multilocularis in Europe. Parasitol. Res. 114, 3167–3169 (2015).
Paolucci, P. & Bon, M. Mammiferi Terrestri D’Italia. Riconoscimento, Ecologia E Tricologia (WBA Handbooks 11, 2022).
Romig, T. & Wassermann, M. Echinococcus species in wildlife. Int. J. Parasitol. -Parasit Wildl. 23, 1–24 (2024).
Giraudoux, P. et al. Drivers of Echinococcus multilocularis transmission in China: small mammal diversity, landscape or climate? PLoS Negl. Trop. Dis. 7: e2045, 1–12 (2013).
Liccioli, S., Kutz, S. J., Ruckstuhl, K. E. & Massolo, A. Spatial heterogeneity and Temporal variations in Echinococcus multilocularis infections in wild hosts in a North American urban setting. Int. J. Parasitol. 44, 457–465 (2014).
Umhang, G. et al. Gray wolves as sentinels for the presence of Echinococcus spp. And other Gastrointestinal parasites in France. Int. J. Parasitol. -Parasit Wildl. 22, 101–107 (2023).
Cenni, L. et al. Current and future distribution of a parasite with complex life cycle under global change scenarios: Echinococcus multilocularis in Europe. Glob. Chang. Biol. 29, 2436–2449 (2023).
Stählberg, S., Bassi, E., Viviani, V. & Apollonio, M. Quantifying prey selection of Northern and Southern European wolves (Canis lupus). Mamm. Biol. 83, 34–43 (2017).
Guimaraes, N. F. et al. What drives Wolf preference towards wild ungulates? Insights from a multi-prey system in the Slovak Carpathians. PLoS One. 17(6): e0265386, 1–14 (2022).
Lavikainen, A. et al. A phylogeny of members of the family Taeniidae based on the mitochondrial cox1 and nad1 gene data. Parasitology 135, 1457–1467 (2008).
Formenti, N. et al. Molecular identification of cryptic cysticercosis: Taenia Ovis Krabbei in wild intermediate and domestic definitive hosts. J. Helminthol. 92, 203–209 (2018).
Al-Sabi, M. N. S. et al. Reappearance of Taenia Ovis Krabbei muscle cysts in a roe deer (Capreolus capreolus) in Denmark after 60 + years. Vet. Parasitol. 196, 225–229 (2013).
Loos-Frank, B. One or two intermediate hosts in the life cycle of Mesocestoides (Cyclophyllidea, Mesocestoididae)? Parasitol. Res. 77, 726–728 (1991).
Chelladurai, J. R. J. J. & Brewer, M. T. Global prevalence of Mesocestoides infections in animals – A systematic review and meta-analysis. Vet. Parasitol. 298, 109537, 1–18 (2021).
Varcasia, A. et al. Species delimitation based on MtDNA genes suggests the occurrence of new species of Mesocestoides in the mediterranean region. Parasit. Vectors. 11, 1-11 (2018).
Al-Sabi, M., Halasa, T. & Kapel, C. Infections with cardiopulmonary and intestinal helminths and sarcoptic mange in red foxes from two different localities in Denmark. Acta Parasitol. 59(1), 98–107 (2014).
de Macedo, M. R. P. et al. Gastrointestinal helminths of wolves (Canis lupus Linnaeus, 1758) in Piedmont, north-western Italy. J. Helminthol. 94, 1–6 (2020).
Coppola, F. et al. Settlement of a stable wolf pack in a highly anthropic area of Pisan hills: relationship with animal husbandry and hunting in a human–wolf coexistence perspective. Anim. Sci. J. 93(1), e13799, 1–12 (2022).
Tamarozzi, F., Deplazes, P. & Casulli, A. Reinventing the wheel of Echinococcus granulosus sensu Lato transmission to humans. Trends Parasitol. 36, 427–434 (2020).
Santoro, A. et al. Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe. Int. J. Parasitol. 54, 233–245 (2024).