The county saw lows of -9C during the early hours of Tuesday morning, making it colder than Reykjavik in Iceland, which recorded -4C, according to the Met Office.
Five schools were forced to close on Tuesday due to the weather conditions, including Warren School in Lowestoft, Witnesham Primary School in Ipswich, Chalk Hall in Sudbury, and Castle East School in Bungay.
Pot Kiln Primary School in Great Cornard was also shut on Tuesday after suffering issues with its hot water.
In Mildenhall, the Barleycorn Café was unable to open due to issues with heating and a very slippery street outside.
A fresh weather warning for ice has been issued from midnight until 10am on Wednesday.
Suffolk Highways has said that due to road temperatures forecast to drop below freezing all priority one routes will be gritted from 6pm.
A spokesman for Suffolk Highways said that a second run of those routes will take place at 4am due to expected overnight rain.
On Monday snow and ice caused widespread disruption across the county.
Two schools across the county were forced to close as a result of the icy conditions.
An amber cold-health alert issued by the UK Health Security Agency is expected to stay in place until 10am on Friday.
Although sub-zero temperatures are forecast to ease slightly towards the end of the week, freezing conditions are still likely to persist.
Highs are expected to reach two degrees on Tuesday, rising to four degrees on Wednesday.
By Sunday, temperatures are forecast to climb to around eight degrees.