Nimelt m\u00f6\u00f6dus eelmisel aastal 50 aastat esimeste teadusartiklite avaldamisest fluorestsents-korrelatsiooni-spektroskoopiast (FCS). Et selgitada, mida Eesti teadlased hiljuti saavutasid ja miks see oluline on, peab k\u00f5igepealt tutvustama FCS-i t\u00e4hendust, alles seej\u00e4rel teadlaste saavutust.<\/p>\n
FCS-i t\u00f6\u00f6p\u00f5him\u00f5te seisneb andmete salvestamisel v\u00e4ga v\u00e4ikesest ruumalast \u2013 umbes femtoliitrist (kvadriljondik liitrit; v\u00f5rdlusena oleks see kui \u00fcks liivaterake ol\u00fcmpiabasseinis!). T\u00e4nap\u00e4eval kasutab FCS konfokaalmikroskoopi, mis suunab laseri proovile \u2013 olgu selleks siis rakk v\u00f5i muu objekt \u2013 ja signaal kogutaksegi ainult v\u00e4ga v\u00e4ikesest vaatlusruumalast laseri fookuses. Seda meetodit saab kasutada, kui selles ruumalas on vaid m\u00f5ni molekul, mis v\u00f5ib fluorestseeruda. Viimane on teadud molekulide omadus kiirata valgust, kui neid ergastavad footonid. Niiv\u00f5rd v\u00e4ikese molekulide arvu puhul muutub m\u00f5\u00f5detav fluorestsentsi signaal v\u00e4ga m\u00fcraseks. Siiski on v\u00f5imalik seostada signaali suurenemist molekulide saabumisega mikroskoobi fookusesse, muutumisega keemiliste reaktsioonide kaudu v\u00f5i nende kinnitumisega pinnale. P\u00f5him\u00f5tteliselt v\u00f5ib uurida k\u00f5ike, mis l\u00e4bi f\u00fc\u00fcsikalise, keemilise v\u00f5i bioloogilise protsessi muudab molekulide fluorestsentsi, nagu liikumine v\u00f5i reaktsioon.<\/p>\n
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