• Sparrer MN, Hodges NF, Sherman T, VandeWoude S, Bosco-Lauth AM, Mayo CE. Role of spillover and spillback in SARS-CoV-2 transmission and the importance of one health in Understanding the dynamics of the COVID-19 pandemic. J Clin Microbiol. 2023;61(7):e01610–22.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Shackelton LA, Parrish CR, Truyen U, Holmes EC. High rate of viral evolution associated with the emergence of carnivore parvovirus. Proc Natl Acad Sci U S A. 2005;102(2):379–84.

    PubMed 

    Google Scholar     

  • Olival KJ, Daszak P. The ecology of emerging neurotropic viruses. J Neurovirol. 2005;11(5):441–6.

    PubMed 

    Google Scholar     

  • Bennett AJ, Paskey AC, Ebinger A, Pfaff F, Priemer G, Höper D, et al. Relatives of Rubella virus in diverse mammals. Nature. 2020;586(7829):424–8.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Bennett AJ, Paskey AC, Ebinger A, Pfaff F, Priemer G, Höper D, et al. Author correction: relatives of Rubella virus in diverse mammals. Nature. 2020;588(7836):E2–E.

    PubMed 

    Google Scholar     

  • Matiasek K, Pfaff F, Weissenböck H, Wylezich C, Kolodziejek J, Tengstrand S, et al. Mystery of fatal ‘staggering disease’ unravelled: novel Rustrela virus causes severe meningoencephalomyelitis in domestic cats. Nat Commun. 2023;14(1):624.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Pfaff F, Breithaupt A, Rubbenstroth D, Nippert S, Baumbach C, Gerst S, et al. Revisiting Rustrela virus: new cases of encephalitis and a solution to the capsid enigma. Microbiol Spectr. 2022;10(2):e00103–22.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Schulze V, Große R, Fürstenau J, Forth LF, Ebinger A, Richter MT, et al. Borna disease outbreak with high mortality in an alpaca herd in a previously unreported endemic area in Germany. Transbound Emerg Dis. 2020;67(5):2093–107.

    PubMed 

    Google Scholar     

  • Völker H, Nessler, Baumgärtner W. First tick-borne encephalitis in a dog resident in Northern Germany. Berl Munch Tierarztl Wochenschr. 2017;130:114–60.


    Google Scholar     

  • Ebinger A, Santos PD, Pfaff F, Dürrwald R, Kolodziejek J, Schlottau K, et al. Lethal Borna disease virus 1 infections of humans and animals – in-depth molecular epidemiology and phylogeography. Nat Commun. 2024;15(1):7908.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Niller HH, Angstwurm K, Rubbenstroth D, Schlottau K, Ebinger A, Giese S, et al. Zoonotic spillover infections with Borna disease virus 1 leading to fatal human encephalitis, 1999–2019: an epidemiological investigation. Lancet Infect Dis. 2020;20(4):467–77.

    PubMed 

    Google Scholar     

  • Pfeffer SH, Leschnik M. TBE in animals. Chapter 10. In: Dobler G, Erber W, Bröker M, Chitimia-Dobler L, Schmitt HJ, eds. The TBE Book2024.

  • Elsmo EJ, Wünschmann A, Beckmen KB, Broughton-Neiswanger LE, Buckles EL, Ellis J, et al. Highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.4b infections in wild terrestrial Mammals, united States, 2022. Emerg Infect Dis. 2023;29(12):2451–60.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Mirolo M, Anne P, Kathrin AA, Bianca K, Ana R-G, Katharina K, et al. Highly pathogenic avian influenza A virus (HPAIV) H5N1 infection in two European grey seals (Halichoerus grypus) with encephalitis. Emerg Microbes Infections. 2023;12(2):2257810.


    Google Scholar     

  • Thorsson E, Zohari S, Roos A, Banihashem F, Bröjer C, Neimanis A. Highly pathogenic avian influenza A(H5N1) virus in a harbor Porpoise, Sweden. Emerg Infect Dis. 2023;29(4):852–5.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Tammiranta N, Isomursu M, Fusaro A, Nylund M, Nokireki T, Giussani E, et al. Highly pathogenic avian influenza A (H5N1) virus infections in wild carnivores connected to mass mortalities of pheasants in Finland. Infect Genet Evol. 2023;111:105423.

    PubMed 

    Google Scholar     

  • Baechlein C, Kleinschmidt S, Hartmann D, Kammeyer P, Wöhlke A, Warmann T, et al. Neurotropic highly pathogenic avian influenza A(H5N1) virus in red Foxes, Northern Germany. Emerg Infect Dis. 2023;29(12):2509–12.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Voss A, Schlieben P, Gerst S, Wylezich C, Pfaff F, Langner C, et al. Rustrela virus infection – An emerging neuropathogen of red-necked wallabies (Macropus rufogriseus). Transbound Emerg Dis. 2022;69(6):4016–21.

    PubMed 

    Google Scholar     

  • de le Roi M, Puff C, Wohlsein P, Pfaff F, Beer M, Baumgärtner W, et al. Rustrela virus as putative cause of nonsuppurative meningoencephalitis in lions. Emerg Infect Dis. 2023;29(5):1042–5.


    Google Scholar     

  • de le Roi M, Nägler I, Rubbenstroth D, Beer M, Höper D, Barth SA, et al. Retrospective analysis of clustered neuroinflammatory and neurodegenerative diseases in captive lions in the early 1970s. Vet Pathol. 2025;0(0):03009858251335280.


    Google Scholar     

  • Fox KA, Breithaupt A, Beer M, Rubbenstroth D, Pfaff F. Rustrela virus in wild mountain Lion (Puma concolor) with staggering Disease, Colorado, USA. Emerg Infect Dis. 2024;30(8):1664–7.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Weiss V, Weidinger P, Matt J, Weissenbacher-Lang C, Nowotny N, Weissenböck H. Rustrela Virus-Associated encephalomyelitis (‘Staggering Disease’) in cats from Eastern Austria, 1994–2016. Viruses. 2023;15(8):1621.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Thilén E, Rubbenstroth D, Tengstrand S, Pfaff F, Wensman JJ, Ley C. Evidence of Rustrela virus-associated feline staggering disease in Sweden since the 1970s. Acta Vet Scand. 2024;66(1):59.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Nippert S, Rubbenstroth D, Geers JA, Ebinger A, Hoffmann D, Breithaupt A et al. Continuous presence of genetically diverse Rustrela virus lineages in yellow-necked field mouse reservoir populations in Northeastern Germany. Virus Evol. 2023;9(2).

  • Dürrwald R, Kolodziejek J, Weissenböck H, Nowotny N. The bicolored White-Toothed shrew crocidura Leucodon (HERMANN 1780) is an Indigenous host of mammalian Borna disease virus. PLoS ONE. 2014;9(4):e93659.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Nobach D, Bourg M, Herzog S, Lange-Herbst H, Encarnação JA, Eickmann M, et al. Shedding of infectious Borna disease Virus-1 in living bicolored White-Toothed shrews. PLoS ONE. 2015;10(8):e0137018.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Böhmer MM, Haring VC, Schmidt B, Saller FS, Coyer L, Chitimia-Dobler L, et al. One health in action: investigation of the first detected local cluster of fatal Borna disease virus 1 (BoDV-1) encephalitis, Germany 2022. J Clin Virol. 2024;171:105658.

    PubMed 

    Google Scholar     

  • Fürstenau J, Richter MT, Erickson NA, Große R, Müller KE, Nobach D, et al. Borna disease virus 1 infection in alpacas: comparison of pathological lesions and viral distribution to other dead-end hosts. Vet Pathol. 2024;61(1):62–73.

    PubMed 

    Google Scholar     

  • Hausmann J, Schamel K, Staeheli P. CD8 + T lymphocytes mediate Borna disease Virus-Induced immunopathology independently of Perforin. J Virol. 2001;75(21):10460–6.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Richt J, Stitz L, Deschl U, Frese K, Rott R. Borna disease virus-induced meningoencephalomyelitis caused by a virus-specific CD4 + T cell-mediated immune reaction. J Gen Virol. 1990;71(11):2565–73.

    PubMed 

    Google Scholar     

  • Kolodziejek J, Dürrwald R, Herzog S, Ehrensperger F, Lussy H, Nowotny N. Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin. J Gen Virol. 2005;86(2):385–98.

    PubMed 

    Google Scholar     

  • Weissenböck H, Bagó Z, Kolodziejek J, Hager B, Palmetzhofer G, Dürrwald R, et al. Infections of horses and shrews with bornaviruses in upper austria: a novel endemic area of Borna disease. Emerg Microbes Infections. 2017;6(1):1–9.


    Google Scholar     

  • Gelpi E, Preusser M, Laggner U, Garzuly F, Holzmann H, Heinz FX, et al. Inflammatory response in human tick-borne encephalitis: analysis of postmortem brain tissue. J Neurovirol. 2006;12(4):322–7.

    PubMed 

    Google Scholar     

  • Andersson E, Kendall A, Url A, Auer A, Leschnik M. The first RT-qPCR confirmed case of tick-borne encephalitis in a dog in Scandinavia. Acta Vet Scand. 2020;62(1):51.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Conze TM, Bagó Z, Revilla-Fernández S, Schlegel J, Goehring LS, Matiasek K. Tick-Borne encephalitis virus (TBEV) infection in two horses. Viruses. 2021;13(9):1775.

    PubMed 
    PubMed Central 

    Google Scholar     

  • de Heus P, Bagó Z, Weidinger P, Lale D, Trachsel DS, Revilla-Fernández S, et al. Severe neurologic disease in a horse caused by Tick-Borne encephalitis Virus, Austria, 2021. Viruses. 2023;15(10):2022.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Böhm B, Schade B, Bauer B, Hoffmann B, Hoffmann D, Ziegler U, et al. Tick-borne encephalitis in a naturally infected sheep. BMC Vet Res. 2017;13(1):267.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Michelitsch A, Wernike K, Klaus C, Dobler G, Beer M. Exploring the reservoir hosts of Tick-Borne encephalitis virus. Viruses. 2019;11(7):669.

    PubMed 
    PubMed Central 

    Google Scholar     

  • European Food Safety Authority, ECfDPCEURLfA I, Adlhoch C, Fusaro A, Gonzales JL, Kuiken T, et al. Avian influenza overview December 2022 – March 2023. EFSA J. 2023;21(3):e07917.

    PubMed Central 

    Google Scholar     

  • Plaza PI, Gamarra-Toledo V, Euguí JR, Lambertucci SA. Recent changes in patterns of mammal infection with highly pathogenic avian influenza A(H5N1) virus worldwide. Emerg Infect Dis. 2024;30(3):444–52.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Braun J, Mundhenk L, Range F, Gruber AD. Quantitative expression analyses of candidates for alternative anion conductance in cystic fibrosis mouse models. J Cyst Fibros. 2010;9(5):351–64.

    PubMed 

    Google Scholar     

  • Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, et al. Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts. Mol Cell Probes. 2007;21(1):47–55.

    PubMed 

    Google Scholar     

  • Toussaint JF, Sailleau C, Breard E, Zientara S, De Clercq K. Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J Virol Methods. 2007;140(1):115–23.

    PubMed 

    Google Scholar     

  • Schwaiger M, Cassinotti P. Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA. J Clin Virol. 2003;27(2):136–45.

    PubMed 

    Google Scholar     

  • Hoffmann B, Depner K, Schirrmeier H, Beer M. A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J Virol Methods. 2006;136(1):200–9.

    PubMed 

    Google Scholar     

  • Hassan KE, Ahrens AK, Ali A, El-Kady MF, Hafez HM, Mettenleiter TC, et al. Improved subtyping of avian influenza viruses using an RT-qPCR-Based low density array: ‘Riems influenza a typing Array’, version 2 (RITA-2). Viruses. 2022;14(2):415.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Gelpi E, Preusser M, Garzuly F, Holzmann H, Heinz FX, Budka H. Visualization of central European Tick-Borne encephalitis infection in fatal human cases. J Neuropathology Experimental Neurol. 2005;64(6):506–12.


    Google Scholar     

  • Heinz FX, Kunz C. Homogeneity of the structural glycoprotein from European isolates of Tick-borne encephalitis virus: comparison with other flaviviruses. J Gen Virol. 1981;57(2):263–74.

    PubMed 

    Google Scholar     

  • Firsching TC, Dietert K, Bartel A, Doherr MG, Gruber AD. Dependence of the Ki67 labelling index of selected canine tumours on patient Age, sex and tumour size. J Comp Pathol. 2022;193:1–8.

    PubMed 

    Google Scholar     

  • Pfaff F, Breithaupt A, Ulrich RG, Beer M, Rubbenstroth D. Rustrela-Virus – neu identifizierter enzephalitiserreger Mit Breitem wirtsspektrum Bei Säugetieren. Berl Munch Tierarztl Wochenschr. 2025;138:1–12.


    Google Scholar     

  • Kronevi T, Nordström M, Moreno W, Nilsson PO. Feline ataxia due to nonsuppurative meningoencephalomyelitis of unknown aetiology. Nord Vet Med. 1974;26(12):720–5.

    PubMed 

    Google Scholar     

  • Nowotny N, Weissenböck H. Description of feline nonsuppurative meningoencephalomyelitis (staggering disease) and studies of its etiology. J Clin Microbiol. 1995;33(6):1668–9.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Weissenböck H, Nowotny N, Zoher J. Feline meningo-encephalomyelitis (staggering disease) in Austria. Wiener Tierärztliche Monatsschrift. 1994;81(7):195–201. ref. 19.


    Google Scholar     

  • Caplazi P, Ehrensperger F. Spontaneous Borna disease in sheep and horses: immunophenotyping of inflammatory cells and detection of MHC-I and MHC-II antigen expression in Borna encephalitis lesions. Vet Immunol Immunopathol. 1998;61(2):203–20.

    PubMed 

    Google Scholar     

  • Caplazi P, Waldvogel A, Stitz L, Braun U, Ehrensperger F. Borna disease in naturally infected cattle. J Comp Pathol. 1994;111(1):65–72.

    PubMed 

    Google Scholar     

  • Metzler A, Ehrensperger F, Wyler R. Natürliche Bornavirus-Infektion Bei Kaninchen. Zentralblatt für Veterinärmedizin Reihe B. 1978;25(2):161–4.


    Google Scholar     

  • Bornand J, Fatzer R, Melzer K, Jmaa DG, Caplazi P, Ehrensperger F. A case of Borna disease in a Cat. Eur J Veterinary Pathol. 1998;4(3):33–5. ref. 13.


    Google Scholar     

  • Ellenberger C, Heenemann K, Vahlenkamp TW, Grothmann P, Herden C, Heinrich A. Borna disease in an adult free-ranging Eurasian beaver (Castor fiber albicus). J Comp Pathol. 2024;209:31–5.

    PubMed 

    Google Scholar     

  • Weissenböck H, Nowotny N, Caplazi P, Kolodziejek J, Ehrensperger F. Borna disease in a dog with lethal meningoencephalitis. J Clin Microbiol. 1998;36(7):2127–30.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Weissenböck H, Suchy A, Holzmann H. Tick-borne encephalitis in dogs: neuropathological findings and distribution of antigen. Acta Neuropathol. 1998;95(4):361–6.

    PubMed 

    Google Scholar     

  • Pfeffer M, Dobler G. Tick-borne encephalitis virus in dogs – is this an issue? Parasites Vectors. 2011;4(1):59.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Im JH, Baek J-H, Durey A, Kwon HY, Chung M-H, Lee J-S. Geographic distribution of Tick-borne encephalitis virus complex. J Vector Borne Dis. 2020;57(1).

  • Hellenbrand W, Kreusch T, Böhmer MM, Wagner-Wiening C, Dobler G, Wichmann O, et al. Epidemiology of Tick-Borne encephalitis (TBE) in Germany, 2001–2018. Pathogens. 2019;8(2):42.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Robert-Koch-Institut. Epidemiologisches Bulletin 09/25. 2025.

  • Süss J, Schrader C, Falk U, Wohanka N. Tick-borne encephalitis (TBE) in Germany — Epidemiological data, development of risk areas and virus prevalence in field-collected ticks and in ticks removed from humans. Int J Med Microbiol Supplements. 2004;293:69–79.


    Google Scholar     

  • Priestnall SL, Schöniger S, Ivens PA, Eickmann M, Brachthäuser L, Kehr K, et al. Borna disease virus infection of a horse in great Britain. Vet Rec. 2011;168(14):380b.

    PubMed 

    Google Scholar     

  • Klopfleisch R, Wolf PU, Uhl W, Gerst S, Harder T, Starick E, et al. Distribution of lesions and antigen of highly pathogenic avian influenza virus A/Swan/Germany/R65/06 (H5N1) in domestic cats after presumptive infection by wild birds. Vet Pathol. 2007;44(3):261–8.

    PubMed 

    Google Scholar     

  • Peacock TP, Moncla L, Dudas G, VanInsberghe D, Sukhova K, Lloyd-Smith JO, et al. The global H5N1 influenza panzootic in mammals. Nature. 2025;637(8045):304–13.

    PubMed 

    Google Scholar     

  • Leguia M, Garcia-Glaessner A, Muñoz-Saavedra B, Juarez D, Barrera P, Calvo-Mac C, et al. Highly pathogenic avian influenza A (H5N1) in marine mammals and seabirds in Peru. Nat Commun. 2023;14(1):5489.

    PubMed 
    PubMed Central 

    Google Scholar     

  • Consortium ENETWILD, Flavia O, Sascha K, Carola S-L, Christoph S, Valerie A, et al. The role of mammals in avian influenza: a review. EFSA Supporting Publications. 2024;21(3):8692E.


    Google Scholar     

  • First tick-borne. Encephalitis in a dog resident in Northern Germany. Berl Munch Tierarztl Wochenschr. 2017;130:114–60.


    Google Scholar     

  • Voss A, Enders J, Dökel S, Breithaupt A, Rubbenstroth D, Mundhenk L. Das Rustrela-Virus Bei nicht-eitrigen Meningoenzephalitiden – weitere Fälle Im Großraum Berlin. Tierarztl Prax Ausg K Kleintiere Heimtiere. 2024;52(03):P19.


    Google Scholar