{"id":107132,"date":"2025-10-07T18:44:14","date_gmt":"2025-10-07T18:44:14","guid":{"rendered":"https:\/\/www.europesays.com\/ie\/107132\/"},"modified":"2025-10-07T18:44:14","modified_gmt":"2025-10-07T18:44:14","slug":"lineage-specific-expansions-of-the-dicer-gene-family-in-tardigrades-bmc-genomics","status":"publish","type":"post","link":"https:\/\/www.europesays.com\/ie\/107132\/","title":{"rendered":"Lineage-specific expansions of the Dicer gene family in tardigrades | BMC Genomics"},"content":{"rendered":"
  • \n

    Bernstein E, Caudy AA, Hammond SM, Hannon GJ. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature [Internet]. 2001;409(6818):363\u20136. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/11201747<\/a><\/p>\n<\/li>\n

  • \n

    Denli AM, Hannon GJ. RNAi: an ever-growing puzzle. Trends Biochem Sci [Internet]. 2003;28(4):196\u2013201. Available from: https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0968000403000586<\/a><\/p>\n<\/li>\n

  • \n

    Sidahmed A, Abdalla S, Mahmud S, Wilkie B. Antiviral innate immune response of RNA interference. The Journal of Infection in Developing Countries [Internet]. 2014;8(07):804\u201310. Available from: https:\/\/jidc.org\/index.php\/journal\/article\/view\/25022288<\/a><\/p>\n<\/li>\n

  • \n

    Heigwer F, Port F, Boutros M. RNA Interference (RNAi) Screening in Drosophila. Genetics [Internet]. 2018;208(3):853\u201374. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5844339\/<\/a><\/p>\n<\/li>\n

  • \n

    Shabalina SA, Koonin EV. Origins and evolution of eukaryotic RNA interference. Trends Ecol Evol [Internet]. 2008;23(10):578\u201387. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2695246\/<\/a><\/p>\n<\/li>\n

  • \n

    Ding SW, Han Q, Wang J, Li WX. Antiviral RNA interference in mammals. Curr Opin Immunol [Internet]. 2018;54:109\u201314. Available from: https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0952791518300803<\/a><\/p>\n<\/li>\n

  • \n

    de Jong D, Eitel M, Jakob W, Osigus HJ, Hadrys H, DeSalle R, et al. Multiple dicer genes in the early-diverging Metazoa. Mol Biol Evol. 2009;26(6):1333\u201340. https:\/\/doi.org\/10.1093\/molbev\/msp042<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Zapletal D, Kubicek K, Svoboda P, Stefl R. Dicer structure and function: conserved and evolving features. EMBO Rep [Internet]. 2023;24(7):e57215. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10328071\/<\/a><\/p>\n<\/li>\n

  • \n

    Welker NC, Maity TS, Ye X, Aruscavage PJ, Krauchuk AA, Liu Q et al. Dicer\u2019s helicase domain discriminates dsRNA termini to promote an altered reaction mode. Mol Cell [Internet]. 2011;41(5):589\u201399. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3061311\/<\/a><\/p>\n<\/li>\n

  • \n

    Sinha NK, Trettin KD, Aruscavage PJ, Bass BL. Drosophila Dicer-2 cleavage is mediated by helicase- and dsRNA termini-dependent states that are modulated by Loquacious-PD. Mol Cell [Internet]. 2015;58(3):406\u201317. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4433149\/<\/a><\/p>\n<\/li>\n

  • \n

    MacKay CR, Wang JP, Kurt-Jones EA. Dicer\u2019s role as an antiviral: still an enigma. Curr Opin Immunol [Internet]. 2014;0:49\u201355. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3932008\/<\/a><\/p>\n<\/li>\n

  • \n

    Ciechanowska K, Pokornowska M, Kurzy\u0144ska-Kokorniak A. Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases. Int J Mol Sci [Internet]. 2021;22(2):616. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7827160\/<\/a><\/p>\n<\/li>\n

  • \n

    Hansen SR, Aderounmu AM, Donelick HM, Bass BL. Dicer\u2019s Helicase Domain: A Meeting Place for Regulatory Proteins. Cold Spring Harb Symp Quant Biol [Internet]. 2019;84:185\u201393. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7384945\/<\/a><\/p>\n<\/li>\n

  • \n

    Ma E, MacRae IJ, Kirsch JF, Doudna JA. Auto-inhibition of Human Dicer by its Internal Helicase Domain. J Mol Biol [Internet]. 2008;380(1):237\u201343. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2927216\/<\/a><\/p>\n<\/li>\n

  • \n

    Grimson A, Srivastava M, Fahey B, Woodcroft BJ, Chiang HR, King N et al. Early origins and evolution of microRNAs and Piwi-interacting RNAs in animals. Nature [Internet]. 2008;455(7217):1193\u20137. Available from: https:\/\/www.nature.com\/articles\/nature07415<\/a><\/p>\n<\/li>\n

  • \n

    Mukherjee K, Campos H, Kolaczkowski B. Evolution of Animal and Plant Dicers: Early Parallel Duplications and Recurrent Adaptation of Antiviral RNA Binding in Plants. Mol Biol Evol [Internet]. 2013;30(3):627\u201341. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3563972\/<\/a><\/p>\n<\/li>\n

  • \n

    Jia H, Kolaczkowski O, Rolland J, Kolaczkowski B. Increased affinity for RNA targets evolved early in animal and plant dicer lineages through different structural mechanisms. Mol Biol Evol. 2017;34(12):3047\u201363. https:\/\/doi.org\/10.1093\/molbev\/msx187<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Formaggioni A, Cavalli G, Hamada M, Sakamoto T, Plazzi F, Passamonti M. The Evolution and Characterization of the RNA Interference Pathways in Lophotrochozoa. Genome Biol Evol [Internet]. 2024;16(5):1\u201318. Available from: https:\/\/doi.org\/10.1093\/gbe\/evae098<\/a><\/p>\n<\/li>\n

  • \n

    Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ et al. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA\/miRNA silencing pathways. Cell [Internet]. 2004;117(1):69\u201381. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15066283<\/a><\/p>\n<\/li>\n

  • \n

    Mapalo MA, Wolfe JM, Ortega-Hern\u00e1ndez J. Cretaceous amber inclusions illuminate the evolutionary origin of tardigrades. Commun Biol. 2024;7(1):953. https:\/\/doi.org\/10.1038\/s42003-024-06643-2<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Giacomelli M, Vecchi M, Guidetti R, Rebecchi L, Donoghue PCJ, Lozano-Fernandez J et al. CAT-Posterior Mean Site Frequencies Improves Phylogenetic Modeling Under Maximum Likelihood and Resolves Tardigrada as the Sister of Arthropoda Plus Onychophora. Lanfear R, editor. Genome Biol Evol [Internet]. 2025;17(1):1\u201314. Available from: https:\/\/doi.org\/10.1093\/gbe\/evae273<\/a><\/p>\n<\/li>\n

  • \n

    Laumer CE, Fern\u00e1ndez R, Lemer S, Combosch D, Kocot KM, Riesgo A, et al. Revisiting metazoan phylogeny with genomic sampling of all phyla. Proc Biol Sci. 2019;286(1906):20190831. https:\/\/doi.org\/10.1098\/rspb.2019.0831<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Schokraie E, Hotz-Wagenblatt A, Warnken U, Frohme M, Dandekar T, Schill RO, et al. Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics. J Zool Syst Evol Res. 2011;49(s1):111\u20139. https:\/\/doi.org\/10.1111\/j.1439-0469.2010.00608.x<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Nelson DR, Bartels PJ, Guil N. Tardigrade Ecology. In: Schill RO, editor. Water Bears: The Biology of Tardigrades [Internet]. Cham: Springer International Publishing; 2018. pp. 163\u2013210. Available from: https:\/\/doi.org\/10.1007\/978-3-319-95702-9_7<\/a><\/p>\n<\/li>\n

  • \n

    Buda J, Olszanowski Z, Wierzgo\u0144 M, Zawierucha K. Tardigrades and oribatid mites in bryophytes from geothermally active lava fields (Krafla, Iceland) and the description of Pilatobius islandicus sp. nov. (Eutardigrada) [Internet]. 2018. Available from: https:\/\/rebus.us.edu.pl\/handle\/20.500.12128\/7700<\/a><\/p>\n<\/li>\n

  • \n

    Romano IIIF, Gallo M, D\u2019Addabbo R, Accogli G, Baguley J, Montagna P. Deep-sea tardigrades in the northern Gulf of Mexico with a description of a new species of Coronarctidae (Tardigrada: Arthrotardigrada), Coronarctus mexicus. Journal of Zoological Systematics and Evolutionary Research [Internet]. 2011;49(s1):48\u201352. Available from: https:\/\/onlinelibrary.wiley.com\/doi\/abs\/<\/a>https:\/\/doi.org\/10.1111\/j.1439-0469.2010.00597.x<\/a><\/p>\n<\/li>\n

  • \n

    Tenlen JR, McCaskill S, Goldstein B. RNA interference can be used to disrupt gene function in tardigrades. Dev Genes Evol [Internet]. 2013;223(3):171\u201381. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23187800<\/a><\/p>\n<\/li>\n

  • \n

    Giovannini I, Boothby TC, Cesari M, Goldstein B, Guidetti R, Rebecchi L. Production of reactive oxygen species and involvement of bioprotectants during anhydrobiosis in the tardigrade Paramacrobiotus spatialis. Sci Rep [Internet]. 2022;12(1):1938. Available from: https:\/\/www.nature.com\/articles\/s41598-022-05734-6<\/a><\/p>\n<\/li>\n

  • \n

    Schill RO, Steinbr\u00fcck G. Identification and differentiation of heterotardigrada and eutardigrada species by riboprinting. J Zool Syst Evol Res. 2007;45(3):184\u201390. https:\/\/doi.org\/10.1111\/j.1439-0469.2007.00409.x<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    M\u00f8bjerg N, J\u00f8rgensen A, Kristensen RM, Neves RC. Morphology and Functional Anatomy. In: Schill RO, editor. Water Bears: The Biology of Tardigrades [Internet]. Cham: Springer International Publishing; 2018. pp. 57\u201394. Available from: https:\/\/doi.org\/10.1007\/978-3-319-95702-9_2<\/a><\/p>\n<\/li>\n

  • \n

    Kamilari M, J\u00f8rgensen A, Schi\u00f8tt M, M\u00f8bjerg N. Comparative transcriptomics suggest unique molecular adaptations within tardigrade lineages. BMC Genomics. 2019;20(1):607.<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Stec D, Krzywa\u0144ski \u0141, Arakawa K, Michalczyk \u0141. A new redescription of Richtersius coronifer, supported by transcriptome, provides resources for describing concealed species diversity within the monotypic genus Richtersius (Eutardigrada). Zool Lett. 2020;6(1):2. https:\/\/doi.org\/10.1186\/s40851-020-0154-y<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Boothby TC, Tapia H, Brozena AH, Piszkiewicz S, Smith AE, Giovannini I et al. Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation. Mol Cell [Internet]. 2017;65(6):975\u2013984.e5. Available from: https:\/\/doi.org\/10.1016\/j.molcel.2017.02.018<\/a><\/p>\n<\/li>\n

  • \n

    Anoud M, Delagoutte E, Helleu Q, Brion A, Duvernois-Berthet E, As M et al. Comparative transcriptomics reveal a novel tardigrade-specific DNA-binding protein induced in response to ionizing radiation. Elife [Internet]. 2024;13:1\u201329. Available from: https:\/\/elifesciences.org\/articles\/92621<\/a><\/p>\n<\/li>\n

  • \n

    Yoshida Y, Koutsovoulos G, Laetsch DR, Stevens L, Kumar S, Horikawa DD, et al. Comparative genomics of the tardigrades Hypsibius dujardini and Ramazzottius varieornatus. PLoS Biol. 2017;15(7):e2002266. https:\/\/doi.org\/10.1371\/journal.pbio.2002266<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Mapalo MA, Arakawa K, Baker CM, Persson DK, Mirano-Bascos D, Giribet G. The unique antimicrobial recognition and signaling pathways in tardigrades with a comparison across ecdysozoa. G3 Genes|Genomes|Genetics. 2020;10(3):1137\u201348.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Murai Y, Yagi-Utsumi M, Fujiwara M, Tanaka S, Tomita M, Kato K et al. Multiomics study of a heterotardigrade, Echinisicus testudo, suggests the possibility of convergent evolution of abundant heat-soluble proteins in Tardigrada. BMC Genomics [Internet]. 2021;22(1):813. Available from: http:\/\/biorxiv.org\/content\/early\/2020\/10\/28\/2020.10.27.358333.abstract<\/a><\/p>\n<\/li>\n

  • \n

    Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30(4):772\u201380. https:\/\/doi.org\/10.1093\/molbev\/mst010<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Criscuolo A, Gribaldo S. BMGE (block mapping and gathering with entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments. BMC Evol Biol. 2010;10(1):210. https:\/\/doi.org\/10.1186\/1471-2148-10-210<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, et al. IQ-tree 2: new models and efficient methods for phylogenetic inference in the genomic era. Mol Biol Evol. 2020;37(5):1530\u20134. https:\/\/doi.org\/10.1093\/molbev\/msaa015<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods [Internet]. 2017;14(6):587\u20139. Available from: https:\/\/www.nature.com\/articles\/nmeth.4285<\/a><\/p>\n<\/li>\n

  • \n

    Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010;59(3):307\u201321. https:\/\/doi.org\/10.1093\/sysbio\/syq010<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS. UFboot2: improving the ultrafast bootstrap approximation. Mol Biol Evol. 2018;35(2):518\u201322. https:\/\/doi.org\/10.1093\/molbev\/msx281<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Lartillot N, Rodrigue N, Stubbs D, Richer J, PhyloBayes MPI. Phylogenetic Reconstruction with Infinite Mixtures of Profiles in a Parallel Environment. Syst Biol [Internet]. 2013;62(4):611\u20135. Available from: https:\/\/doi.org\/10.1093\/sysbio\/syt022<\/a><\/p>\n<\/li>\n

  • \n

    Manni M, Berkeley MR, Seppey M, Sim\u00e3o FA, Zdobnov EM. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Kelley J, editor. Mol Biol Evol [Internet]. 2021;38(10):4647\u201354. Available from: https:\/\/academic.oup.com\/mbe\/article\/38\/10\/4647\/6329644<\/a><\/p>\n<\/li>\n

  • \n

    Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND. Nat Methods [Internet]. 2015;12(1):59\u201360. Available from: https:\/\/www.nature.com\/articles\/nmeth.3176<\/a><\/p>\n<\/li>\n

  • \n

    Herranz M, Stiller J, Worsaae K, S\u00f8rensen MV. Phylogenomic analyses of mud dragons (Kinorhyncha). Mol Phylogenet Evol [Internet]. 2022;168(December 2021):107375. Available from: https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1055790321003080<\/a><\/p>\n<\/li>\n

  • \n

    Sato S, Cunha TJ, de Medeiros BAS, Khost DE, Sackton TB, Giribet G. Sizing Up the Onychophoran Genome: Repeats, Introns, and Gene Family Expansion Contribute to Genome Gigantism in Epiperipatus broadwayi. Vieira C, editor. Genome Biol Evol [Internet]. 2023;15(3):1\u20136. Available from: https:\/\/doi.org\/10.1093\/gbe\/evad021<\/a><\/p>\n<\/li>\n

  • \n

    Lord A, Cunha TJ, de Medeiros BAS, Sato S, Khost DE, Sackton TB et al. Expanding on Our Knowledge of Ecdysozoan Genomes: A Contiguous Assembly of the Meiofaunal Priapulan Tubiluchus corallicola. Wheat C, editor. Genome Biol Evol [Internet]. 2023;15(6):1\u20136. Available from: https:\/\/doi.org\/10.1093\/gbe\/evad103<\/a><\/p>\n<\/li>\n

  • \n

    Wang HC, Minh BQ, Susko E, Roger AJ. Modeling site heterogeneity with posterior mean site frequency profiles accelerates accurate phylogenomic estimation. Syst Biol. 2018;67(2):216\u201335. https:\/\/doi.org\/10.1093\/sysbio\/syx068<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Lu S, Wang J, Chitsaz F, Derbyshire MK, Geer RC, Gonzales NR et al. CDD\/SPARCLE: the conserved domain database in 2020. Nucleic Acids Res [Internet]. 2020;48(D1):D265\u20138. Available from: https:\/\/doi.org\/10.1093\/nar\/gkz991<\/a><\/p>\n<\/li>\n

  • \n

    Mirdita M, Sch\u00fctze K, Moriwaki Y, Heo L, Ovchinnikov S, Steinegger M. ColabFold: making protein folding accessible to all. Nat Methods [Internet]. 2022;19(6):679\u201382. Available from: https:\/\/www.nature.com\/articles\/s41592-022-01488-1<\/a><\/p>\n<\/li>\n

  • \n

    Sehnal D, Bittrich S, Deshpande M, Svobodov\u00e1 R, Berka K, Bazgier V et al. Mol* Viewer: modern web app for 3D visualization and analysis of large biomolecular structures. Nucleic Acids Res [Internet]. 2021;49(W1):W431\u20137. Available from: https:\/\/academic.oup.com\/nar\/article\/49\/W1\/W431\/6270780<\/a><\/p>\n<\/li>\n

  • \n

    Grant BJ, Rodrigues APC, ElSawy KM, McCammon JA, Caves LSD. Bio3d: an R package for the comparative analysis of protein structures. Bioinformatics [Internet]. 2006;22(21):2695\u20136. Available from: https:\/\/academic.oup.com\/bioinformatics\/article\/22\/21\/2695\/252414<\/a><\/p>\n<\/li>\n

  • \n

    Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res [Internet]. 2004;32(5):1792\u20137. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15034147<\/a><\/p>\n<\/li>\n

  • \n

    Yoshida Y, Sugiura K, Tomita M, Matsumoto M, Arakawa K. Comparison of the transcriptomes of two tardigrades with different hatching coordination. BMC Dev Biol. 2019;19(1):1\u20139.<\/p>\n

    Article<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Krueger F, Babraham Institute. 2015. Trim Galore! A wrapper tool around Cutadapt and FastQC to consistently apply quality and adapter trimming to FastQ files. Available from: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/trim_galore\/<\/a><\/p>\n<\/li>\n

  • \n

    Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29(1):15\u201321. https:\/\/doi.org\/10.1093\/bioinformatics\/bts635<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Pertea G, Pertea M. GFF Utilities: GffRead and GffCompare. F1000Res [Internet]. 2020;9:ISCB Comm J-304. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7222033\/<\/a><\/p>\n<\/li>\n

  • \n

    Liao Y, Smyth GK, Shi W. Featurecounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinformatics. 2014. https:\/\/doi.org\/10.1093\/bioinformatics\/btt656<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Horton NJ, Kleinman K, Using. R and RStudio for Data Management, Statistical Analysis, and Graphics [Internet]. CRC Press; 2015. 280 p. Available from: https:\/\/books.google.com\/books?id=W1G3BgAAQBAJ<\/a><\/p>\n<\/li>\n

  • \n

    Vergani-Junior CA, Tonon-da-Silva G, Inan MD, Mori MA. DICER: structure, function, and regulation. Biophys Rev. 2021;13(6):1081\u201390. https:\/\/doi.org\/10.1007\/s12551-021-00902-w<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ et al. Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA\/miRNA Silencing Pathways. Cell [Internet]. 2004;117(1):69\u201381. Available from: https:\/\/www.cell.com\/cell\/abstract\/S0092-8674(04)00261-2<\/a><\/p>\n<\/li>\n

  • \n

    Kidwell MA, Chan JM, Doudna JA. Evolutionarily Conserved Roles of the Dicer Helicase Domain in Regulating RNA Interference Processing. Journal of Biological Chemistry [Internet]. 2014;289(41):28352\u201362. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4192488\/<\/a><\/p>\n<\/li>\n

  • \n

    Kaufman EJ, Miska EA. The microRNAs of Caenorhabditis elegans. Semin Cell Dev Biol [Internet]. 2010;21(7):728\u201337. Available from: https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1084952110001060<\/a><\/p>\n<\/li>\n

  • \n

    Lim LP, Lau NC, Weinstein EG, Abdelhakim A, Yekta S, Rhoades MW, et al. The microRNAs of Caenorhabditis elegans. Genes Dev. 2003;17(8):991\u20131008. https:\/\/doi.org\/10.1101\/gad.1074403<\/a>.<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Bukhari SIA, Vasquez-Rifo A, Gagn\u00e9 D, Paquet ER, Zetka M, Robert C et al. The microRNA pathway controls germ cell proliferation and differentiation in C. elegans. Cell Res [Internet]. 2012;22(6):1034\u201345. Available from: https:\/\/www.nature.com\/articles\/cr201231<\/a><\/p>\n<\/li>\n

  • \n

    McMenamin AJ, Daughenbaugh KF, Flenniken ML. The Heat Shock Response in the Western Honey Bee (Apis mellifera) is Antiviral. Viruses [Internet]. 2020;12(2):245. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7077298\/<\/a><\/p>\n<\/li>\n

  • \n

    Aderounmu AM, Aruscavage PJ, Kolaczkowski B, Bass BL. Ancestral protein reconstruction reveals evolutionary events governing variation in Dicer helicase function. Elife [Internet]. 2023;12:e85120. Available from: https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC10159624\/<\/a><\/p>\n<\/li>\n","protected":false},"excerpt":{"rendered":"Bernstein E, Caudy AA, Hammond SM, Hannon GJ. Role for a bidentate ribonuclease in the initiation step of…\n","protected":false},"author":2,"featured_media":107133,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_share_on_mastodon":"0"},"categories":[77],"tags":[2569,18,67095,910,19,17,3544,9693,6720,67093,67094,6719,3549,13166,133,42962],"class_list":["post-107132","post","type-post","status-publish","format-standard","has-post-thumbnail","category-science","tag-animal-genetics-and-genomics","tag-eire","tag-gene-duplication","tag-general","tag-ie","tag-ireland","tag-life-sciences","tag-microarrays","tag-microbial-genetics-and-genomics","tag-n-dicern","tag-phylogenetics","tag-plant-genetics-and-genomics","tag-proteomics","tag-rnai","tag-science","tag-tardigrades"],"share_on_mastodon":{"url":"","error":""},"_links":{"self":[{"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/posts\/107132","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/comments?post=107132"}],"version-history":[{"count":0,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/posts\/107132\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/media\/107133"}],"wp:attachment":[{"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/media?parent=107132"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/categories?post=107132"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.europesays.com\/ie\/wp-json\/wp\/v2\/tags?post=107132"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}