Customer of 50 years Jo Cunningham said she would miss the shop “unbelievably”.
“There is nothing like Pet Needs anywhere else on the Wirral,” she said.
“This is an amazing, unique shop. The personal service, the deliveries, the advice, you can just pop in whatever your need to ask them and they are here.”
Another customer Chris Fog said: “They are so knowledgeable in what they do.
“There is a personal sense of nothing is too much trouble for them and I’d like to think that they’ve become friends as well.”
She described the closure as “the end of an era” and wished the couple a “wonderful retirement”.