This is the third Gromit Unleashed trail – the first was unveiled in 2013.
The trails have raised more than £20m for The Grand Appeal to date, thanks to auction sales and donations.
Nicola Masters, director of The Grand Appeal, said: “It’s the one and only time to see all 53 large sculptures together before they go off to auction.”
She told BBC Radio Bristol that 100 Norbot sculptures painted by local pupils will also be displayed before returning to schools.
Four miniature Wallace, Gromit and Feathers McGraw artefacts, currently hidden within Bristol Museum & Art Gallery, will also be on display.
“People are being incredibly generous when they’re out and about on the trail,” Ms Masters said.
“It’s for a really serious cause.”