{"id":824439,"date":"2026-03-14T03:54:16","date_gmt":"2026-03-14T03:54:16","guid":{"rendered":"https:\/\/www.europesays.com\/uk\/824439\/"},"modified":"2026-03-14T03:54:16","modified_gmt":"2026-03-14T03:54:16","slug":"plasmids-promote-antimicrobial-resistance-through-insertion-sequence-mediated-gene-inactivation","status":"publish","type":"post","link":"https:\/\/www.europesays.com\/uk\/824439\/","title":{"rendered":"Plasmids promote antimicrobial resistance through insertion sequence-mediated gene inactivation"},"content":{"rendered":"
  • \n

    Ho, C. S. et al. Antimicrobial resistance: a concise update. Lancet Microbe 6<\/b>, 100947 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Okeke, I. N. et al. The scope of the antimicrobial resistance challenge. Lancet 403<\/b>, 2426\u20132438 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Kariuki, S. Global burden of antimicrobial resistance and forecasts to 2050. Lancet 404<\/b>, 1172\u20131173 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Alekshun, M. N. & Levy, S. B. Molecular mechanisms of antibacterial multidrug resistance. Cell 128<\/b>, 1037\u20131050 (2007).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Darby, E. M. et al. Molecular mechanisms of antibiotic resistance revisited. Nat. Rev. Microbiol. 21<\/b>, 280\u2013295 (2023).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    MacLean, R. C. & San Millan, A. The evolution of antibiotic resistance. Science 365<\/b>, 1082\u20131083 (2019).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    San Millan, A. Evolution of plasmid-mediated antibiotic resistance in the clinical context. Trends Microbiol. 26<\/b>, 978\u2013985 (2018).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Shepherd, M. J. et al. Ecological and evolutionary mechanisms driving within-patient emergence of antimicrobial resistance. Nat. Rev. Microbiol. 22<\/b>, 650\u2013665 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Brito, I. L. Examining horizontal gene transfer in microbial communities. Nat. Rev. Microbiol. 19<\/b>, 442\u2013453 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Casta\u00f1eda-Barba, S., Top, E. M. & Stalder, T. Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era. Nat. Rev. Microbiol. 22<\/b>, 18\u201332 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Hawkey, P. M. & Jones, A. M. The changing epidemiology of resistance. J. Antimicrob. Chemother. 64<\/b>, i3\u2013i10 (2009).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Mathers, A. J., Peirano, G. & Pitout, J. D. The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae. Clin. Microbiol. Rev. 28<\/b>, 565\u2013591 (2015).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Rodr\u00edguez-Beltr\u00e1n, J., DelaFuente, J., Le\u00f3n-Sampedro, R., MacLean, R. C. & San Mill\u00e1n, \u00c1 Beyond horizontal gene transfer: the role of plasmids in bacterial evolution. Nat. Rev. Microbiol. 19<\/b>, 347\u2013359 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Jordt, H. et al. Coevolution of host\u2013plasmid pairs facilitates the emergence of novel multidrug resistance. Nat. Ecol. Evol. 4<\/b>, 863\u2013869 (2020).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Dunn, S., Carrilero, L., Brockhurst, M. & McNally, A. Limited and strain-specific transcriptional and growth responses to acquisition of a multidrug resistance plasmid in genetically diverse Escherichia coli lineages. mSystems 6<\/b>, e00083-21 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Toribio-Celestino, L. et al. A plasmid-chromosome crosstalk in multidrug resistant enterobacteria. Nat. Commun. 15<\/b>, 10859 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Thompson, C. M. A. et al. Plasmids manipulate bacterial behaviour through translational regulatory crosstalk. PLoS Biol. 21<\/b>, e3001988 (2023).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Hall, R. J., Snaith, A. E., Thomas, M. J. N., Brockhurst, M. A. & McNally, A. Multidrug resistance plasmids commonly reprogram the expression of metabolic genes in Escherichia coli. mSystems 9<\/b>, e0119323 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Hernandez-Beltran, J. C. R. et al. Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria. Nat. Commun. 15<\/b>, 2610 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Rodriguez-Beltran, J. et al. Multicopy plasmids allow bacteria to escape from fitness trade-offs during evolutionary innovation. Nat. Ecol. Evol. 2<\/b>, 873\u2013881 (2018).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Ramiro-Mart\u00ednez, P. et al. Plasmid mutation rates scale with copy number. Proc. Natl Acad. Sci. USA 123<\/b>, e2526088123 (2026).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Remigi, P. et al. Transient hypermutagenesis accelerates the evolution of legume endosymbionts following horizontal gene transfer. PLoS Biol. 12<\/b>, e1001942 (2014).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Stoesser, N. et al. Evolutionary history of the global emergence of the Escherichia coli epidemic clone ST131. mBio https:\/\/doi.org\/10.1128\/mbio.02162-15<\/a> (2016).<\/p>\n<\/li>\n

  • \n

    Peirano, G., Chen, L., Kreiswirth, B. N. & Pitout, J. D. D. Emerging antimicrobial-resistant high-risk Klebsiella pneumoniae clones ST307 and ST147. Antimicrob. Agents Chemother. https:\/\/doi.org\/10.1128\/aac.01148-20<\/a> (2020).<\/p>\n<\/li>\n

  • \n

    Le\u00f3n-Sampedro, R. et al. Pervasive transmission of a carbapenem resistance plasmid in the gut microbiota of hospitalized patients. Nat. Microbiol. 6<\/b>, 606\u2013616 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Babakhani, S. & Oloomi, M. Transposons: the agents of antibiotic resistance in bacteria. J. Basic Microbiol. 58<\/b>, 905\u2013917 (2018).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Roy, P. H. & Partridge, S. R. in Antimicrobial Drug Resistance: Mechanisms of Drug Resistance Vol. 1 (eds Mayers D. L. et al.) 61\u201376 (Springer, 2017).<\/p>\n<\/li>\n

  • \n

    Siguier, P., Gourbeyre, E. & Chandler, M. Bacterial insertion sequences: their genomic impact and diversity. FEMS Microbiol. Rev. 38<\/b>, 865\u2013891 (2014).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Yao, Y. et al. Intra- and interpopulation transposition of mobile genetic elements driven by antibiotic selection. Nat. Ecol. Evol. 6<\/b>, 555\u2013564 (2022).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Sami, H., Khan, P. A. & Singh, A. in Beta-Lactam Resistance in Gram-Negative Bacteria: Threats and Challenges (eds Shahid, M. et al.) 169\u2013178 (2022).<\/p>\n<\/li>\n

  • \n

    Vandecraen, J., Chandler, M., Aertsen, A. & Van Houdt, R. The impact of insertion sequences on bacterial genome plasticity and adaptability. Crit. Rev. Microbiol. 43<\/b>, 709\u2013730 (2017).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Xie, F. et al. Large-scale genomic analysis reveals significant role of insertion sequences in antimicrobial resistance of Acinetobacter baumannii. mBio 16<\/b>, e02852-24 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Grieshop, M. P. et al. Replicative selfish genetic elements are driving rapid pathogenic adaptation of Enterococcus faecium. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2025.03.16.643550<\/a> (2025).<\/p>\n<\/li>\n

  • \n

    Li, X.-Z., Elkins, C. A. & Zgurskaya, H. I. Efflux-Mediated Antimicrobial Resistance in Bacteria: Mechanisms, Regulation and Clinical Implications (Springer, 2016).<\/p>\n<\/li>\n

  • \n

    Delcour, A. H. Outer membrane permeability and antibiotic resistance. Biochim. Biophys. Acta 1794<\/b>, 808\u2013816 (2009).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Schaenzer, A. J. & Wright, G. D. Antibiotic resistance by enzymatic modification of antibiotic targets. Trends Mol. Med. 26<\/b>, 768\u2013782 (2020).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Xie, M. et al. Increasing polymyxin resistance in clinical carbapenem-resistant Klebsiella pneumoniae strains in China between 2000 and 2023. Commun. Med. 5<\/b>, 73 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Yang, T.-Y. et al. Contributions of insertion sequences conferring colistin resistance in Klebsiella pneumoniae. Int. J. Antimicrob. Agents 55<\/b>, 105894 (2020).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Cannatelli, A. et al. MgrB inactivation is a common mechanism of colistin resistance in KPC-producing Klebsiella pneumoniae of clinical origin. Antimicrob. Agents Chemother. 58<\/b>, 5696\u20135703 (2014).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Ghasemian, A., Shafiei, M., Hasanvand, F. & Shokouhi Mostafavi, S. K. Carbapenem and colistin resistance in Enterobacteriaceae: worldwide spread and future perspectives. Rev. Med. Microbiol. 29<\/b>, 173\u2013176 (2018).<\/p>\n

    Article<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    El-Mahallawy, H. A., El Swify, M., Abdul Hak, A. & Zafer, M. M. Increasing trends of colistin resistance in patients at high-risk of carbapenem-resistant Enterobacteriaceae. Ann. Med. 54<\/b>, 2748\u20132756 (2022).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Ha, Y. et al. Transposon-plasmid nesting enables fast response to fluctuating environments. Preprint at bioRxiv https:\/\/doi.org\/10.1101\/2025.06.04.657954<\/a> (2025).<\/p>\n<\/li>\n

  • \n

    Duan, E. S., Kosterlitz, O. & Kerr, B. Agents of change: a partnership between mobile genetic elements facilitates rapid bacterial adaptation. Trends Microbiol. 32<\/b>, 1156\u20131158 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Poirel, L., Bonnin, R. A. & Nordmann, P. Genetic features of the widespread plasmid coding for the carbapenemase OXA-48. Antimicrob. Agents Chemother. 56<\/b>, 559\u2013562 (2012).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Sastre-Dominguez, J. et al. Plasmid-encoded insertion sequences promote rapid adaptation in clinical enterobacteria. Nat. Ecol. Evol. 8<\/b>, 2097\u20132112 (2024).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Luria, S. E. & Delbr\u00fcck, M. Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28<\/b>, 491\u2013511 (1943).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Zheng, Q. rSalvador: an R package for the fluctuation experiment. G3 7<\/b>, 3849\u20133856 (2017).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Goldstein, B. P. Resistance to rifampicin: a review. J. Antibiot. 67<\/b>, 625\u2013630 (2014).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Kato, A., Tanabe, H. & Utsumi, R. Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters. J. Bacteriol. 181<\/b>, 5516\u20135520 (1999).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Bonin, N. et al. MEGARes and AMR++, v3.0: an updated comprehensive database of antimicrobial resistance determinants and an improved software pipeline for classification using high-throughput sequencing. Nucleic Acids Res. 51<\/b>, D744\u2013D752 (2023).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Durrant, M. G., Li, M. M., Siranosian, B. A., Montgomery, S. B. & Bhatt, A. S. A bioinformatic analysis of integrative mobile genetic elements highlights their role in bacterial adaptation. Cell Host Microbe 27<\/b>, 140\u2013153.e9 (2020).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Coluzzi, C. & Rocha, E. P. C. The spread of antibiotic resistance is driven by plasmids among the fastest evolving and of broadest host range. Mol. Biol. Evol. 42<\/b>, msaf060 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Hern\u00e1ndez-Garc\u00eda, M. et al. Characterization of carbapenemase-producing Enterobacteriaceae from colonized patients in a university hospital in Madrid, Spain, during the R-GNOSIS project depicts increased clonal diversity over time with maintenance of high-risk clones. J. Antimicrob. Chemother. 73<\/b>, 3039\u20133043 (2018).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Alonso-del Valle, A. et al. Antimicrobial resistance level and conjugation permissiveness shape plasmid distribution in clinical enterobacteria. Proc. Natl Acad. Sci. USA 120<\/b>, e2314135120 (2023).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Le Roux, F., Binesse, J., Saulnier, D. & Mazel, D. Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector. Appl. Environ. Microbiol. 73<\/b>, 777\u2013784 (2007).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Wand, M. E. & Sutton, J. M. Mutations in the two component regulator systems PmrAB and PhoPQ give rise to increased colistin resistance in Citrobacter and Enterobacter spp. J. Med. Microbiol. 69<\/b>, 521\u2013529 (2020).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Sommer, H., Schumacher, B. & Saedler, H. A new type of IS1-mediated deletion. Mol. Gen. Genet. 184<\/b>, 300\u2013307 (1981).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Reif, H. J. & Saedler, H. IS1 is involved in deletion formation in the gal region of E. coli K12. Mol. Gen. Genet. 137<\/b>, 17\u201328 (1975).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Quon, H. et al. Quantifying conjugation rates in clinical and environmental matrices: a systematic review to inform risk assessment. Front. Microbiomes https:\/\/doi.org\/10.3389\/frmbi.2024.1490240<\/a> (2025).<\/p>\n<\/li>\n

  • \n

    Fordham, S. M. E., Mantzouratou, A. & Sheridan, E. Prevalence of insertion sequence elements in plasmids relating to mgrB gene disruption causing colistin resistance in Klebsiella pneumoniae. Microbiologyopen 11<\/b>, e1262 (2022).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Shankar, C., Pragasam, A. K., Anandan, S. & Veeraraghavan, B. mgrB as hotspot for insertion sequence integration: change over from multidrug-resistant to extensively drug-resistant Klebsiella pneumoniae? Microb. Drug Resist. 25<\/b>, 1122\u20131125 (2019).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Chen, X. et al. Insertion sequence mediating mrgB disruption is the major mechanism of polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae isolates from China. J. Glob. Antimicrob. Resist. 30<\/b>, 357\u2013362 (2022).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Rajakani, S. G. et al. Characterization of genome-wide transpositions induced by colistin exposure in multi-drug-resistant Klebsiella pneumoniae. Antimicrob. Agents Chemother. 69<\/b>, e0157424 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Siguier, P., Gourbeyre, E., Varani, A., Ton-Hoang, B. & Chandler, M. Everyman\u2019s guide to bacterial insertion sequences. In Mobile DNA III (eds Chandler, M. et al.) 555\u2013590 (John Wiley & Sons, 2015).<\/p>\n<\/li>\n

  • \n

    Che, Y. et al. Conjugative plasmids interact with insertion sequences to shape the horizontal transfer of antimicrobial resistance genes. Proc. Natl Acad. Sci. USA 118<\/b>, e2008731118 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Tenaillon, O., Taddei, F., Radman, M. & Matic, I. Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation. Res. Microbiol. 152<\/b>, 11\u201316 (2001).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Andersson, D. I. & Hughes, D. Selection and transmission of antibiotic-resistant bacteria. Microbiol. Spectr. https:\/\/doi.org\/10.1128\/microbiolspec.mtbp-0013-2016<\/a> (2017).<\/p>\n<\/li>\n

  • \n

    Consuegra, J. et al. Insertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria. Nat. Commun. 12<\/b>, 980 (2021).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Sousa, A., Bourgard, C., Wahl, L. M. & Gordo, I. Rates of transposition in Escherichia coli. Biol. Lett. 9<\/b>, 20130838 (2013).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Dewar, A. E., Belcher, L. J. & West, S. A. A phylogenetic approach to comparative genomics. Nat. Rev. Genet. 26<\/b>, 395\u2013405 (2025).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Razavi, M., Kristiansson, E., Flach, C.-F. & Larsson, D. G. J. The association between insertion sequences and antibiotic resistance genes. mSphere https:\/\/doi.org\/10.1128\/msphere.00418-20<\/a> (2020).<\/p>\n<\/li>\n

  • \n

    Touchon, M. & Rocha, E. P. C. Causes of insertion sequences abundance in prokaryotic genomes. Mol. Biol. Evol. 24<\/b>, 969\u2013981 (2007).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Datsenko, K. A. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl Acad. Sci. USA 97<\/b>, 6640\u20136645 (2000).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    DelaFuente, J. et al. Within-patient evolution of plasmid-mediated antimicrobial resistance. Nat. Ecol. Evol. 6<\/b>, 1980\u20131991 (2022).<\/p>\n

    Article<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Dahlberg, C., Bergstr\u00f6m, M. & Hermansson, M. In situ detection of high levels of horizontal plasmid transfer in marine bacterial communities. Appl. Environ. Microbiol. 64<\/b>, 2670\u20132675 (1998).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Deatherage, D. E. & Barrick, J. E. Identification of mutations in laboratory evolved microbes from next-generation sequencing data using breseq. Methods Mol. Biol. 1151<\/b>, 165\u2013188 (2014).<\/p>\n

    Article<\/a>\u00a0
    \n     CAS<\/a>\u00a0
    \n     PubMed<\/a>\u00a0
    \n     PubMed Central<\/a>\u00a0
    \n
    \n Google Scholar<\/a>\u00a0\n <\/p>\n<\/li>\n

  • \n

    Rodera-Fernandez, P., Fuentes-Hernandez, A. & Sastre-Dom\u00ednguez, J. MDR_IS: plasmids promote antimicrobial resistance through insertion sequence-mediated gene inactivation. Zenodo https:\/\/doi.org\/10.5281\/zenodo.17885110<\/a> (2025).<\/p>\n<\/li>\n","protected":false},"excerpt":{"rendered":"Ho, C. S. et al. Antimicrobial resistance: a concise update. Lancet Microbe 6, 100947 (2025). Article\u00a0 PubMed\u00a0 Google…\n","protected":false},"author":2,"featured_media":824440,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_share_on_mastodon":"0"},"categories":[8],"tags":[26068,180114,3968,6552,12308,6554,11476,6553,70,16,15,40369],"class_list":["post-824439","post","type-post","status-publish","format-standard","has-post-thumbnail","category-science","tag-antimicrobial-resistance","tag-clinical-microbiology","tag-general","tag-infectious-diseases","tag-life-sciences","tag-medical-microbiology","tag-microbiology","tag-parasitology","tag-science","tag-uk","tag-united-kingdom","tag-virology"],"share_on_mastodon":{"url":"https:\/\/pubeurope.com\/@uk\/116225511616630379","error":""},"_links":{"self":[{"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/posts\/824439","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/comments?post=824439"}],"version-history":[{"count":0,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/posts\/824439\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/media\/824440"}],"wp:attachment":[{"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/media?parent=824439"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/categories?post=824439"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.europesays.com\/uk\/wp-json\/wp\/v2\/tags?post=824439"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}