Imaging of nanoscale polar textures in quantum paraelectric SrTiO3
Cochran, W. Crystal stability and the theory of ferroelectricity. Adv. Phys. 9, 387–423 (1960). Article ADS CAS Google…
Small-molecule modulation of β-arrestins | Nature
Cell culture, antibodies, and reagents HEK-293 cells (ATCC), including transient and stable lines, as well as CRISPR–Cas9 βARR1/2-knockout…
Structural basis for chaperone-guided assembly of RNA-induced silencing complex
Iwasaki, S. et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol. Cell 39,…
Substrate selectivity of the human RNA m5C methyltransferase NSUN2
Roundtree, I. A., Evans, M. E., Pan, T. & He, C. Dynamic RNA modifications in gene expression regulation.…
β-Arrestin condensates regulate G-protein-coupled receptor function
Generation of constructs Constructs were developed using a modified overlap cloning technique60. Flexible linkers, consisting of glycine–serine repeats…
SNOR promotes translation restart after dormancy
Sample preparation for cryo-ET Cryo-ET samples were prepared using 200-mesh R2/2 copper grids (Quantifoil) plasma-cleaned for 30 s in…
Myosin forces remodel F-actin for mechanosensitive protein recognition
Unless otherwise noted, chemicals and buffer components were purchased from Sigma-Aldrich. Cell culture PtK2 cells (ATCC, CCL-56) were…
Electric dipole moment drives the dynamics of the TNFR1 complex I signalosome
Micheau, O. & Tschopp, J. Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 114,…
Structures of Marburgvirus glycoprotein and its complex with NPC1 receptor
Cell lines and plasmids HEK293T and Huh7 cells (American Type Culture Collection (ATCC)) were maintained in Dulbecco’s modified…
DICER cleavage fidelity is governed by 5′-end binding pockets
Plasmid construction The pXG-10×His-DICER plasmid was generated by inserting a DNA sequence encoding human DICER (amino acids 25–1922)…